Equipment

Overview

The TAMIC facility is equipped with two state-of-the-art Leica laser scanning confocal/multiphoton systems (TCS SP8) as well as a confocal Leica TCS SP2 microscope. The spectral imaging modalities covered by this suite of instruments include:
 

  • Confocal reflectance & fluorescence
  • Differential interference contrast (DIC)
  • Two-photon excited fluorescence (TPEF)
  • Second harmonic generation (SHG)
  • Coherent anti-Stokes Raman spectroscopy (CARS)
  • Fluorescence lifetime imaging (FLIM)
     

Other features available for enhanced microscopic imaging include:
 

  • Resonant scanning (16 kHz line scan; 28 frames/s for 512x512 frame)
  • Dispersion compensation for multiphoton imaging
  • HyD detectors (low-noise, high quantum yield PMT/avalanche photodiode hybrid)
  • Image stitching, microplate imaging and adaptive focus
  • Environmental chamber and automatic water dispenser for long-term & live cell studies
  • Continuous zoom range of 0.75-48x
  • Motorized stages (resolution=0.04µm, reproducibility<1µm)